Project
CMV + GFP

Part:BBa_K1852000

Designed by: Manuel Lerma   Group: iGEM15_Tec-Chihuahua   (2015-09-18)


GFP + CMV Promoter used for expression in oocyte cells. This part was planned to be used as a report

It is compose of the BBa_K747096 which is the CMV promotor and BBa_E0040 which is the GFP, we used for know if our oocytes were well transfected, the CMV promoter is for eukaryotic cells and the GFP is the mark.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 576
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1230


[edit]
Categories
//chassis/eukaryote
//function/reporter
Parameters
chassisB. taurus
functionReporter
proteinGFP